Isolation and molecular identification of Klebsiella pneumonia from different human samples in Anbar province
Keywords:
Klebsiella pneumonia, Urine, Burns, WoundAbstract
This study aimed to isolate and molecular identifying of Klebsiella pneumonia from different human samples in Anbar province. Patients sent to clinics in the province of Anbar with burns, and wounds of all ages and gender s provided a total of 92 samples. The samples were collected using sterile, single-use cotton swabs, while urine samples were taken by tube and centrifuged and take from sediment, then all stored in BHI broth, and then cultured for 24 hours at 37°C on MacConkey agar. The suspected isolates were diagnosed according to microbiological reference. The DNA of suspected bacteria were extracted by using of kit purchased from Geneaid company, the extraction process was carried out in accordance with the manufacturer’s instructions. Two genes were examined among the suspected isolates, these were 16S rRNA and rmpA. Two hours of electrophoresis were carried out using a 70 volt/35 mAmp current. With the use of a UV transilluminator, the DNA bands were seen and captured on camera. The results demonstrate that 12(13%) K. pneumoniae isolates were found from 92 clinical samples, with 8 (66.7%) coming from urine, 3 (25%) from burns, and 1 (8.3%) from a wound. The antibiotic sensitivity test showed that the highest sensitivity rate of K. pneumonia was recorded to imipenem (70%), gentamycin (65%), ciprofloxacin (60%) and streptomycin (50%), while the lowest sensitivity rate was recorded to tetracycline (20%) and trimethoprim (30%). The 16S rRNA gene was used for the molecular identification of all isolates (12). When the rmpA primer was used to amplify 12 K. pneumoniae isolates, 3 isolates (25%) tested positive for the rmpA gene. In conclusion, Klebsiella pneumonia was isolated at significant point especially from patient with UTI and these isolates were confirmed molecularly by using 16S rRNA gene.
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References
R. Ranjbar, H. Memariani, R. Sorouri, and M.
Memariani, “Distribution of virulence genes
and genotyping of CTX-M- 15-producing
Klebsiella pneumoniae isolated from patients
with community-acquired urinary tract
infection (CA-UTI),” Microbial Pathogenesis,
vol. 100, pp. 244-249, 2016.
R. Ranjbar, S. S. Mostafavi, and H. Memariani,
“Evaluation of multiple-locus variable-number
tandem repeat analysis (MLVA) for genotyping of
Escherichia coli isolated from Karaj river,” Water
Supply, vol. 18, no. l,pp. 160—166,2017.
M. Hasannejad-Bibalan, M.Sadeghi, H. Hemmati et al.,
“A two-year study of microbiological characteristics
of intravascular catheter-related bloodstream
infections at Razi hospital, Iran,” New Zealand
Journal of Medical Laboratory Science, vol. 75, no.
,pp. 202-205,2021.
M. Kashefieh, H. Hosainzadegan, S. Baghbanijavid, andR. Ghotaslou, “(e molecular epidemiology of
resistance to antibiotics among Klebsiella
pneumoniae isolates in Azerbaijan, Iran,” Journal of
Tropical Medicine, vol. 2021, Article ID 9195184, 9
pages, 2021.
M. Halaji, S. Shahidi, B. Ataei, A. Atapour, A. Feizi,
andS. A. Havaei, “Molecular epidemiology of
blaCTX-M geneproducing uropathogenic
Escherichia coli among Iraniankidney transplant
patients: clonal dissemination of CC131 andCClO,”
Annalsof Clinical Microbiology and Antimicrobials,
vol. 20, no. 1, p. 65, 2021.
M. Halaji, A. Feizi, A. Mirzaei et al., “(e global
prevalence ofclass 1 integron and associated
antibiotic resistance mEscherichia coli from
patients with urinary tract infections,
asystematic review and meta-analysis,”
Microbial Drug Resistance, vol. 26, no. 10, pp.
-1218,2020.
A. Sadeghi, M. Halaji, A. Fayyazi, and S. A. Havaei,
“Characterization of plasmid-mediated quinolone
resistance and serogroup distributions of
uropathogenic Escherichia coli among Iranian
kidney transplant patients,” BioMed
Researchlntemational, vol. 2020, Article ID
, 7 pages, 2020.
J. Gou, N. Liu, L. Guo et al., “Carbapenem-resistant
Enterobacter hormaechei STI 103 with IMP-26
carbapenemase andESBL gene blaSHV-178,”
Infection and Drug Resistance, vol. 13, pp. 597—605,
F. Adzitey, N. Huda, and G. R. R. Ali, “Molecular
techniquesfor detecting and typing of bacteria,
advantages and application to foodbome pathogens
isolatedfrom ducks,”3Biotech,vol. 3, no. 2, pp. 97—
, 2013.
MacFaddin, J.F. (2000). Biochemical tests for
identification of medical bacteria. Lippincott
Williams & Wilkins. Philadelphia, USA.
Turton, J.; Baklan, H.; Siu, L.; Kaufmann, M. and
Pitt, T. (2008). Evaluation of a multiplex per for
detection of serotypes kl , k2 and k5 in Klebsiella
spp. and comparison of isolates within these
serotypes. FEMS Microbiol. Lett., 284: 247-
CLSI-Clinical and L. S. Institute, Performance
Standards for Antimicrobial Susceptibility Testing
th Informational Supplement (M100-S30) 2020,
Clinical and Laboratory Standards Institute, Wayne,
IL, USA, 2020.
SAS. (2012). Statistical Analysis System, User's
Guide. Statistical. Version 9.1th ed. SAS. Inst.
Inc. Cary. N.C. USA.
Hansen, D. (1997). Clinical and Bacteriological
Characterization of Klebsiella Bacteraemia. Ph.D.
thesis. Copenhagen University, Denmark.
Abdul Razzaq, M.; Trad, J. and Al-Maamory, E.
(2013). Genotyping and detection of some
virulence genes of Klebsiella pneumonia isolated
from clinical cases. Med. J. of Babylon, 10 (2):
-399.
Omar-Zahid, L. (2009). Extraction and purification of
the Klebocin from clinically isolated Klebsiella and
studying its biochemical and biological
characteristics. M.Sc. thesis. Baghdad University,
College of Science.
Petit, P.; schneeberger, P.; lidala, V.; Butter, M. and
Wamola, I. (1991). Bacteriology of infections in
aural tropical area of Kenya: isolates and antibiotic
susceptibility, (abs). East. Afr. Med. J., 68 (7): 500-
Lafta, A. (2010). Klebocin effect partially purified
from bacteria Klebsiella pneumoniae isolated
locally on some intestinal bacteria and some
immune cells. M.Sc. thesis. Al-Mustansiriyah
University, College of Science, Department of
Biology.
Al-dhahri, N. (2002). Klebsiella infections and drug
resistance to antibiotics. M.Sc. thesis. Damascus
University, College of Medical, Syria
Al-Hilfi, S. (2009). The sensitivity of the Escherichia
coli isolated from urinary infections to the two
current antibiotic Ciprofloxacin and
Lomefloxian. M.Sc. thesis. Al-Mustansiriyah
University, College of Science, Department of
Biology.
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