Urinary 302bmicroRNAs gene expression in recurrent lower urinary tract infection caused by Pseudomonas aeruginosa Isolates.
Keywords:
Gene expression 302b, microRNA, mRNAgene, Real-time PCR, RUTI.Abstract
The real-time polymerase chain reactions were performed by using specific primers with reference gene GAPDH and the target genes 302b microRNA, A case—control study was done in Babylon city hospital from February 2021 to March 2022. A total of 110 patients with RUTIs with different age and sex, and healthy individuals as control group were enrolled in this study. Midstream urine was taken for culturing and identification of Pseudomonas aeruginosa.The present study found that the expression of Mi302b gene expression increased in the RUTI patient with P. aeruginosa when compared with control group.so the expression gene is increased more than (%25)fold when compare with control group.which considered as a potential biomarker ,the result show (30) 27.3% isolate were belong to P. aeruginosawhere able to manage chronic and/or recurrent infections represents a significant difference at P<0.05 .Method RNA was extracted from patients group and controls ,then gene expression of miR-302b by relative RT-PCRboth in patient and control.
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