The relationship of Mirna 155 with the target gene SOCS1 in Rheumatoid arthritis in Iraqi patients
Keywords:
miRNA155gene, SOCS1 , RAAbstract
The organs of the body are affected by the chronic inflammatory disease rheumatoid arthritis (RA) in which related with autoimmune disease (AID). The purpose of this research was to study the miRNA155 molecule and its related with the target SOCS1 gene in RA patients and to determine the range of miRNA155 gene expression in the diagnosis of RA, in order to assess the potential diagnostic significance of the circulating miRNA155 gene as a biomarker in RA patients. The total of study was included 14 healthy people for comparison with 36 samples of RA patients, ranging in age from 20 to 60. The patients with RA were diagnosed and tested clinically and immunologically under supervision of the rheumatologist unit in Baghdad Educational Hospital/Altib city/ Baghdad-Iraq. Relative quantification (RQ) of miRNA155 expression was performed after miRNA extraction from the serum of all study participants, was estimated using RT- qPCR. Significant Findings observed an increased in the expression of the miRNA155 profile based on the folding test (11. 12-fold expression 1.08) in the patients with RA, in comparison to healthy individuals, as well as the increased of the target gene (SOCS1) expression through the control compared to the patients whom were at risk showed a highly significant (P≤0.01) in expression levels in which assessed by ELISA technique. In conclusion, the study discovered increased levels of miRNA155 in the blood and depression in the target gene (SOCS1), which may serve as prognostic indicators as a biomarker for illness.
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