Molecular expression of inflammatory cytokines (interleukin IL-1B, IL-6, IL-10 and INF- among patients with breast cancer
Keywords:
breast cancer, PCR, IL-1B, IL-6, IL-10 and INF-ɤAbstract
The total number of breast cancer patients involved in this study are (80) individual were taken from oncology center in Basrah province, the age of patients range from 27-76 yrs and (80) individuals considered as control group after they were checked and confirmed to be free from any chest or any other health problems. the maximum number of breast cancer females was found within the age groups 50-59 yrs (32.5%), fand the lowest rate in age group 20-29 (2.5%), with P =0.0163. PCR amplification results which was done on the extracted DNA have been confirmed by using gel electrophoresis which appear as compact DNA separated bands which were results from the accurate and specific binding between the target DNA template for IL-1β and its specific primer, only bands with expected molecular size 249bp were observed. The target DNA template for IL-6 and its specific primer, these bands viewed with specific size 408pb. the target DNA template for IL-10 and its specific primer, these bands viewed with specific size 295pb. The target DNA template for IFN-γ and its specific primer, these bands viewed with specific size 262 pb
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References
Andre, F., Dieci, M. V., Dubsky, P., Sotiriou, C., Curigliano, G.,
Denkert, C., & Loi, S. (2013). Molecular pathways:
involvement of immune pathways in the therapeutic
response and outcome in breast cancer. Clinical cancer
research, 19(1), 28-33.
Dmitrieva, O. S., Shilovskiy, I. P., Khaitov, M. R., &
Grivennikov, S. I. (2016). Interleukins 1 and 6 as main
mediators of inflammation and cancer. Biochemistry
(Moscow), 81(2), 80-90.
Ernst, M., & Jenkins, B. J. (2004). Acquiring signalling
specificity from the cytokine receptor gp130. Trends in
genetics, 20(1), 23-32.
Fasoulakis, Z., Kolios, G., Papamanolis, V., & Kontomanolis,
E. N. (2018). Interleukins associated with breast cancer.
Cureus, 10(11).
Filippi, I., Carraro, F., & Naldini, A. (2015). Interleukin-1
affects MDAMB231 breast cancer cell migration under
hypoxia: role of HIF-1 and NF B transcription factors.
Mediators of inflammation.
Fourkala, E. O. (2011). Risk factors and novel biomarkers in
breast cancer (Doctoral dissertation, UCL (University
College London)).
He, Z. L., Yang, X. E., & Stoffella, P. J. (2005). Trace elements
in agroecosystems and impacts on the environment.
Journal of Trace elements in Medicine and Biology, 19(2-
, 125-140.
Hoeijmakers J.H.(2009). DNA damage, aging, and cancer, N.
Engl. J. Med. 8;361(15):1475-85.
Joshi, B. H., Leland, P., Lababidi, S., Varrichio, F., & Puri, R. K.
(2014). Interleukin-4 receptor alpha overexpression in
human bladder cancer correlates with the pathological
grade and stage of the disease. Cancer medicine, 3(6),
-1628.
Karakus, N., Kara, N., Ulusoy, A. N., Ozaslan, C., & Bek, Y.
(2011). Tumor necrosis factor alpha and beta and
interferon gamma gene polymorphisms in Turkish breast
cancer patients. DNA and cell biology, 30(6), 371-377.
Kim, T. H., Kim, H. R., & Myung, S. C. (2011). Detection of
nanobacteria in patients with chronic prostatitis and
vaginitis by reverse transcriptase polymerase chain
reaction. Korean journal of urology, 52(3), 194-199.
Kong, F., Liu, J., Liu, Y., Song, B., Wang, H., & Liu, W. (2010).
Association of interleukin-10 gene polymorphisms with
breast cancer in a Chinese population. Journal of
Experimental & Clinical Cancer Research, 29(1),1-7.
Lacroix, M., & Leclercq, G. (2005). The "portrait" of
hereditary breast cancer. Breast cancer research and
treatment, 89(3), 297-304.
Lee, H. L., Lee, K. M., Son, S. J., Hwang, S. H., & Cho, H. J.
(2004). Temporal expression of cytokines and their
receptors mRNAs in a neuropathic pain model.
Neuroreport, 15(18), 2807-2811.
Mocellin, S., Marincola, F. M., & Young, H. A. (2005).
Interleukin-10 and the immune response against cancer:
a counterpoint. Journal of leukocyte biology, 78(5),1043-
Naldini, A., Filippi, I., Miglietta, D., Moschetta, M., Giavazzi,
R., & Carraro, F. (2010). Interleukin-1 regulates the
migratory potential of MDAMB231 breast cancer cells
through the hypoxia-inducible factor-1 . Europ. J.
Cancer, 46(18), 3400-3408.
Pravica, V., Perrey, C., Stevens, A., Lee, J. H., & Hutchinson, I.
V. (2000). A single nucleotide polymorphism in the first
intron of the human IFN- gene:: Absolute correlation
with a polymorphic CA microsatellite marker of high IFNproduction. Human immunology, 61(9), 863-866.
Ruiz, G. M., Fofonoff, P. W., Carlton, J. T., Wonham, M. J., &
Hines, A. H. (2000). Invasion of coastal marine
communities in North America: apparent patterns,
processes, and biases. Annual review of ecology and
systematics, 31(1), 481-531.
Tegoshi, H., Hasegawa, G., Obayashi, H., Nakano, K.,
Kitagawa, Y., Fukui, M., ... & Yoshikawa, T. (2002).
Polymorphisms of interferon- gene CA-Repeat and
interleukin-10 promoter region (-592A/C) in Japanese
type I diabetes. Human immunology, 63(2),121-128.
Zhou, L; Zheng, D., Wang, S., Zhu, J., Jia, Y., Sun, D., Xu, J.,
Wang, Q., Chen, H., Xu, F., Li, B., & Ye, L. (2016). Genetic
association of Toll- like receptor 4 gene and coronary
artery disease in a Chinese Han population. SpringerPlus,
(1), 1533.
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