Effect of Capparis spinosa Ethanolic Extract on Testosterone induced alopecia in Mice

Abstract

 Androgenetic alopecia (AGA) One of the most prevalent chronic disease observed in dermatologic outpatient departments is male or female pattern hair loss. a disorder that affects more than 80% and 42% of Caucasian men and women over the age of 70 years, respectively. The goal of this study was to see how effective a preparation was at promoting hair growth of the Ethanolic extract of Capparis spinosa on albino mice using a topical testosterone gel- induced alopecia model. Material and Methods : The albino mice were divided into 5 groups 10 mice in each group.:(A) intact negative control (without testosterone) (B) Testosterone gel 1%only (C) Testosterone gel 1% + Finasteride solution (2%) (D) Testosterone gel 1% + Capparis spinosa cream( 10%) (E) Testosterone gel 1% + glycerin cream 15% (vehicle). Topical Testosterone gel (1%) was used to induce alopecia in all intervention groups except negative control group , C.spinosa Cream 10% was applied topically to the back skin of animals in the respective group. Hair growth was assessed using visual observation and histological investigation of multiple skin slices using parameters such as (anagen/telogen ratio , follicle density (number of follicles/mm) , Standard diagnostic test ELISA kits were used to detect testosterone and dihydrotestosterone levels in the serum, according to the manufacturer's recommendations. . Those treated with testosterone showed a patch of diffuse hair loss after 21 days, whereas animals treated with C. spinosa demonstrated less hair loss than others who were only given testosterone. Results: C. Spinosa treated group had a follicular density of 5.6 ± 1.577, compared to 2.4 ± 0.516 in the testosterone group and 3.6 ± 0.966 in the finasteride group .Anagen/telogen ratio was significantly affected by C.spinosa which was 3.3 ± 1.475 as compared with 0.5 ± 0.408 and 2 ± 1.080 for Testosterone and Finasteride groups respectively. Testosterone and dihydrotestosterone serum levels was significantly affected by C. spinosa as compared with testosterone and finasteride treated groups. C.spinosa was discovered to have good action against Testosterone-induced alopecia based on visual observation and quantitative data (follicular density, anagen/telogen ratio, and serum hormones concentration ). Conclusion: C.spinosa, when applied topically, promotes hair growth development and has an anti androgenic action, making it a promising therapy option for male pattern baldness and other androgen dependent condition .