Experimental Study on Early Bone Fracture Healing in The Rabbit Using Bone Graft Combined with Salmon Calcitonin, Immunohistochemical and Histological Outcomes
Keywords:
(Salmon calcitonin, Beta-Tricalcium phosphate, Osteocalcin).Abstract
This study aims to evaluate the histological and immunohistochemical changes after systemic administration of salmon calcitonin with Beta-tricalcium phosphate on fracture healing in rabbits by the quantitive study of the osteoblast’s cells at different stages of early bone healing using osteocalcin antibody. Histological findings for the bone healing process in the treated group with β-tricalcium phosphate and salmon calcitonin showed early bone marrow, trabecular bone, mineralization, and compact bone formation in comparison to the healing process in the control group. Immunohistochemical findings expression of anti-osteocalcin revealed that there was a non-significant value in the control group between (2 weeks mean+SD 0.27±0.06 and 4 weeks mean+SD 0.29±0.06). while the treated group with β- tricalcium phosphate and salmon calcitonin showed highly significant differences at (2 weeks mean+SD 0.5±0.05 and 4 weeks mean+SD 0.73±0.07). The conclusion of this study that salmon calcitonin and β- tricalcium phosphate has a major osteogenesis role for early bone formation in the treated group as compared with the control group. β-tricalcium phosphate and systemic salmon calcitonin 2 IU/kg were more effective in the acceleration of the early bone healing process and enhancement of bone regeneration.
Downloads
References
Arisawa EAL, Rocha RF, Carvalho YR, Moraes E, and Almeida JD,.
InflueAncia da calcitonina na reparacsa~o o'ssea de ti'bias de
ratas ovariectomizadas. Po'sGrad Rev Fac Odontol Sa~o Jose' dos
Campos.
Aulakh TS and Tanaka T. 2009 Long-term clinical outcomes following
the use of synthetic hydroxyapatite and bone graft in impaction in
revision hip arthroplasty.Biomaterials.
Costoff A. 2008 "Sect. 5, Ch. 6: Anatomy, Structure, and Synthesis of
Calcitonin (CT)". Endocrinology: hormonal control of calcium and
phosphate.
Groeneveld EHJ, and Burger EH,. 2000. Bone morphogenetic proteins
in human bone regeneration. Eur J Endocrinol;142: 9-21.
Hironori Sugimoto, Yusuke Inagaki, Akira Furukawa, Tsutomu Kira,
Sachiko Kawasaki, Yoshinobu Uchihara, Manabu Akahane &
Yasuhito Tanaka 2021. p-Tricalcium phosphate and their properties.
Itagaki T, Honma T,and Takahashi I,. 2008. Quantitative analysis
and localization of mRNA transcripts of type I collagen, osteocalcin,
MMP2, MMP8, and MMP13 during bone healing in a rat calvarial
experimental defect model. AnatRec .J. D. Almeida, E. A. L. Arisawa, R. F. da Rocha, and Y. R. Carvalho,. 2007.
Effect of calcitonin on bone regeneration in male rats: a
histomorphometric analysis. Int. J. Oral Maxillofac.
Lee K. C., Park M. O., Na D. H., Youn Y. S., Lee S. D., Yoo S. D., Lee H.
S., DeLuca P. P. 2OO3.Intranasal delivery of PEGylated salmon
calcitonins: hypocalcemic effects in rats. Calcif Tissue.
Matthew B. McLaughlin; Ishwarlal Jialal. 2022, California. 18 July.
Northstate University, VA Medical center, Mather, CA. Calcitonin
study Treasure Island (FL): StatPearls Publishing.
Moore KL, Agur AM. 2009 Clinically Oriented Anatomy, 6th ed,
Williams & Wilkins, Baltimore.
Naot D. and Cornish J. 2008. The role of peptides and receptors of the
calcitonin family in the regulation of bone metabolism. Bone.
Qian Z, Li H, Yang H, Yang Q, Lu Z, Wang L, Chen Y, Li X. 2021.
"Osteocalcin attenuates oligodendrocyte differentiation and
myelination via GPR37 signaling in the mouse brain". Science
Advances.
Sekiguchi, Toshio; Kuwasako, Kenji; Ogasawara, Michio; Takahashi,
Hiroki; Matsubara, Shin; Osugi, Tomohiro; Muramatsu, Ikunobu;
Sasayama, Yuichi; Suzuki, Nobuo; Satake, Honoo 2016. Salmon
calcitonin interview.
Seokhwan Yun, Dami Choi,Dong-Jin Choi,Songwan Jin,Won-Soo Yun,
Jung-Bo Huh, and Jin-Hyung Shim.2021,Bone Fracture-Treatment
Method: Fixing 3D-Printed Polycaprolactone Scaffolds with
Hydrogel Type Bone-Derived Extracellular Matrix and p-Tricalcium
Phosphate as an Osteogenic Promoter. 23.Aug.
Theofilos Karachalios, George P Lyritis, Dionyssis G Giannarakos,
George Papanicolaou and Kostas Sotopoulos. 2009, Calcitonin
effects on rabbit bone 24 Nov.
Vieira EMM; Camila Satie Ferreira Ueno; Vivian Neves Valva; Maria
das Granas Vilela Goulart; Terezinha de Oliveira Nogueira;and
Monica Fernandes Gomes,.2008. Bone regeneration in cranioplasty
and clinical complications in rabbits with alloxaninduced diabetes.
Braz.
Downloads
Published
Issue
Section
License
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
