Gene expression of ogr system in Staphylococcus aureus and it is relation with antibiotic resistance
S. aureus, 16srRNA gene, agr system, antibiotic resistance
Abstract
Background: A commensal bacterium has ability to cause a wide range of infections is Staphylococcus aureus device-related infections by regulator system called accessory gene regulator (agr). Methods: Identification S. aureus that isolated from urine clinical sample has been done according to the cultured on the mannitol salt agar and biochemical tests, then confirm the identification by amplification 16srRNA gene through conventional PCR and detection agr (I, II, III, IV) gene by multiplex PCR then expression of agr gene with antibiotic and without antibiotic by real time (qPCR). Result: VThirty isolates of S. aureus diagnosed and (76,70%) percentage of this isolates resistance to amoxicillin and found all this isolates have agr gene that related with antibiotic resistance. Conclusion: 16sRNA polymerase chain reaction is confirmation method for distinction S. aureus strain from other type of bacteria and agr gene have important role in antibiotic resistance.
Downloads
References
Miller, C and Gilmore, J. (2020). Detection of QuorumSensing Molecules for Pathogenic Molecules Using CellBased and Cell-Free Biosensors. Antibiotics;;9, (259) ;
doi:10.3390/antibiotics9050259.
Le, K.Y and Otto, M. (2015). Quorum- sensing regulation in
staphylococci-an overview. Frontiers in
microbiology,6(1174).
Paulander, W., Varming, A. N., Bojer, M. S., Friberg, C., Baek,
K and lngmer,H. (2018). The agr quorum sensing system
in Staphylococcus aureus cells mediates death of sub¬
population. BMC Research Notes ,11:503 .
Saedi, S., Derakhshan, S and Ghaderi , E. (2020). Antibiotic
resistance and typing of agr locus in Staphylococcus aureus
isolated from clinical samples in Sanandaj, Basic Med Sci,
:1307-1314. doi:10.22038/ijbms.2020.46064.10661.
Gnanamani, A., Hariharan, P., & Paul-Satyaseela, M (2017).
Staphylococcus aureus: Overview of bacteriology, clinical
diseases, epidemiology, antibiotic resistance and therapeutic
approach. Academic Press:Cambridge,MA,USA, .4, 28-36.
Chotigarpa, R., Na Lampang, K., Pikulkaew, S., Okonogi, S.,
Ajariyakhajorn, K., and Mektrirat, R. (2018). 'Inhibitory
effects and killing kinetics of lactic acid rice gel against
pathogenic bacteria causing bovine mastitis', Scientia
pharmaceutica. Multidisciplinary Digital Publishing
Institute, 86(3), 1-10.
Omran, A. S., and Hussein, A. N. (2019). Molecular
investigation of mec gene Among coagulase negative
Staphylococcus isolated from different cases. Journal of
Physics: Conference Series, 1234(1), 12074Rakotovao-Ravahatra, Z. D.( Randriatsarafara, F. M.,
Milasoanjara, R. N., Ranaivosoa, M. K., Rakotovao, A. L.,
and Rasamindrakotroka, A. (2019). Assessment of the
Coagulase Test in the Identification of Staphylococcus
aureus Strains. Journal of Biotechnology and
Biomedicine, 2(3), 105-111.
Guo , Y., Song, G., Sun,M. , Wang,J., and Wang, Y .(2020).
Prevalence and Therapies of Antibiotic- Resistance in
Staphylococcus areus.Forntiers in Cellular and infection
Microbiology. 10;107.
Onasanya, A., Mignouna, HD and Thottappilly, G. (2003).
Genetic fingerprinting and phylogenetic diversity of
Staphylococcus aureus isolates from Nigeria. African
Journal of Biotechnology, 2(8): 246-250.
Al-Noaami, R. J. (2014). Detection the genotype estimation
of allele frequencies for ABO groups an Rh factor in
population of Selah Al-Dine and Kirkuk and Neneva
provinces, Ph.D. thesis , College of Education . Tikrit
University.
Cox, M. J., Cookson, W.O., Moffatt, M. F. (2013). Sequencing
the human microbiome in health and disease. Hum Mol
Genet 22(R1), R88-94.
Gumaa , M. A., Idris, A. B., Bilal , N. E and Hassan, M. A.
(2021). First insights into molecular basis identifcation
of 16 s ribosomal RNA gene of Staphylococcus aureus
isolated from Sudan. Gumaa et al. BMC Res Notes ,
:240 , doi.org/10.1186/s13104-021-05569-w.
Clinical and Laboratory Standaards Institute (CLSI). (2022).
Performance Standard for Antimicrobial Susceptibility
testing .32th ed, West Valley Road, Pennsylvania, USA.
Sastry, A. S., Bhat, S., Janagond, A. B., Deepashree R., Ray,
P., Sistla, S . (2021 ). Essentials of Medical Microbiology.
th ed , JAYPEE BROTHERS Medical Publishers The
Health Sciences Publisher New Delhi | London , ISBN:
-81-947090-1-5.
Fadhil, H and Mohammed, J. (2022). Molecular and Classical
identification of Staphylococcus aureus , isolated from
Iraqi Patients with recurrent tonsillitis . Medico-legal
Update, 22(1).
Zhang, Y., Xu, D., Shi, L., Cai, R., Li, Ch and Yan, H. (2018).
Virulence factors, biofilm formation and antibiotic
resistance of Staphylococcus aureus isolates from prok
production. Frontiers in microbiology, 9:1876.
Al-Zoubi, M., Al-Tayyar, S. I., Ali, H. E., Al Jabali, A. and
Khudairat S. (2015). Antimicrobial susceptibility pattern
of Staphylococcus aureus isolated from clinical
specimens in Northern area of Jordan. Iran. J.
MICROBIAL. 7 (5): 265-272.
Bernabe, G., Dal Pra, M., Ronca, V., Pauletto, A., Marzaro, G.,
Saluzzo, F., et al. (2021). A novel Aza-derivative inhibits
quorum sensing signaling and synergizes MethicillinResistant Staphylococcus aureus to clindamycin. Front.
Microbiol. 12:610859. doi:10.3389/fmicb.2021.610859.
Bibalan, M.H., Shakeri, F., Javid, N., Ghaemi , A., Ghaemi ,
E.A. ( 2018). Accessory Gene Regulator Types of
Staphylococcus aureus Isolated in Gorgan, North of Iran.
Journal of Clinical and Diagnostic Research. 8(4): DC07-
DC09, DOI:10.7860/JCDR/2014/6971.4219
Kalia, V.C. et al. (2019).Quorum sensing inhibitors as
antipathogens: biotechnological applications Biotechnol.
Adv.
Downloads
Published
Issue
Section
License
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation .
No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
