Separation and determination of sulfamethoxazole, trimethoprim and metoclopramide hydrochloride by RP-HPLC method in pure and in Pharmaceutical formulations
Keywords:
High Performance Liquid Chromatography, Sulfamethoxazole,Trimethoprim, and Metoclopramide HClAbstract
A very accurate, fast and simple method for separating and determine pharmaceutical compounds sulfamethoxazole (Sulfa), trimethoprim (Tri), and metacalpermide hydrochloride (Meta). The separation process was performed by HPLC on a C18 stationary phase column. The method was developed and validated. The mobile phase is a mixture of acetonitrile buffer 60.40 under isocratic elution. The flow rate was 1.2 mL/min, and the optimum temperature for the separation was 45 °C. The analysis time is up to 4 minutes with retention times of 2.204, 2.873 and 4.983 minutes for the studied pharmaceutical compounds. The linearity was in the range of 0.1-100 µg/mL. The developed method was tested for the quantitative determination of pharmaceutical compounds in its pure form and in different commercial pharmaceutical formulations with less interference of additives.
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