Phylogenetic analysis of the infectious bronchitis virus in Iraqi farms
Keywords:
IBV, chicken, real-time RT-PCR, and sequencing for the S1 geneAbstract
BV is one of the viral illnesses that occurs most often in the poultry sector, and it is caused by the Gamma Coronavirus genus and the Coronaviridae family. In this experiment, fifty specimens of trachea and lung were taken from various locations inside Iraq (Baghdad, Wasit, Diyala, Salahuddin, and Babylon). The chickens' ages ranged from 12 to 26 days, and they displayed respiratory symptoms such as gasping and tracheal rales. The majority of samples were taken from broiler chickens in the range of morbidity and postmortem examination, such as pus in the trachea. Real-time RT-PCR was used to diagnose IBV, which amplified the S1 gene. The result of the RT-PCR test was positive for eight of the specimens. In order to sequence the S1 gene, the Anicon laboratory in Germany, using the Sanger method, was provided with three samples to analyze. The results of the sequencing of the S1 gene indicated that the isolated copies were related to those of the Iranian strain IS/1494/06 that were placed at the National Center for Biotechnology Information website in the Genbank database.
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