Molecular study of Klebsiella spp. isolated from patients with different clinical isolate
Keywords:
K. pneumonia, Clinical isolates, Antibiogram, DNA sequencing.Abstract
Background: Klebsiella pneumonia is one of the most important pathogen that is responsible for burn infection. The aim of this study was to investigate the prevalence rate of Klebsiella spp. among isolates from inpatients at Al-hosien hospital. Materials and Methods: A total of 100 clinical isolates were collected sequentially in different wards at the Al-Hosien, hospital, Karbala, Iraq, from December 2021 to May 2022. All isolates were diagnosed by biochemical tests and VITEK 2 system, and Klebsiella pneumonia isolates were selected for further research and 16srRNA amplification was done by PCR, and phylogenetic tree was drowned. Results: A total of 32 Klebsiella pneumonia isolates were collected from patients, the antibiogram results showed that, the highest rate of resistance was related to ampicillin (87%), and the lowest rate of resistance was related to amikacin (5%), the analysis of phylogenetic tree showed that, 8 isolates have had same genotype while another isolates have differ genotypes. Conclusion: in summary we showed that K. pneumonia have high prevalence rate among clinical isolates.
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