Developing and Validating a Spectrophotometric Method for Estimating Antifungal (Nystatin) in its Pure Form Pharmaceutical Formulation Using Tetrachloro- 1,4 benzoquinone
Keywords:
UV—VIS absorption spectroscopy, Charge-Transfer Complex, Nystatin (Nys.) & tetrachloro-1,4- benzoquinone (TCBQ).Abstract
The UV—VIS absorption spectroscopy technique was used to study the formation of a new complex of charge transfer (CT) between bioactive organic molecules as (Nystatin) containing both a Ji-electrons from a conjugated system and lone-pair of electrons (amine) with Tetrachloro-1,4 benzoquinone (TCBQ) as a ri-acceptor in which the transferred electron goes into its vacant anti-bonding molecular orbitals. The Tyrian, purple-colored complex formed was quantitatively measured at 544 nm. This complex shows obeying Beer’s law within the concentration range of (10-90) pg.ml-lThe stoichiometry of the formed complex between the (Nys.) and (TCBQ) was found 1:2 as evaluated by continuous variation (Job's method) and mole ratio method The value of molar absorptivity was calculated at 7038.2840 L.mol-l.cm-1, while Sandell’s sensitivity value was estimated to be 0.01315 qg.cm-2, while LOD and LOQ were found to be 0.5661and 1.71558 qg.ml-1, respectively. The Charge-Transfer complex association constant (KCT) value was evaluated using Benesi-Hildebrand equation and was found to be 3.00E+03 L.mol-1. This procedure was successfully involved in the analysis of pharmaceutical formulations.
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