Relationship between viral and bacterial diarrhea in children suffering from the gastroenteritis infection

Authors

  • Ahmed Jwan Ahmed Ali Dept, of Microbiology, College of Medicine, University of Babylon, Iraq. Author
  • Al-Saffar Anwar Kadhim Department of biology. College of Sciences. University of Babylon, Iraq Author

Keywords:

Gastrointestinal (GI), Rotavirus, (Sig-A) secretory immunoglobulin -A, Mobilized Colistin gene MCR-1

Abstract

 Introduction: Foodborne diseases are digestive system infections that are transferred by ingesting certain foods or beverages. Persistent diarrhea can occur in infections that last longer than 2 weeks but less than 4 weeks. The consumption of infected food or water might result in travelers’ diarrhea. Rotavirus commonly causes severe, watery diarrhea and vomiting in infants and young children. Children may become dehydrated and need to be hospitalized and can even die. While in bacterial infection (Multidrug-resistant bacteria causes diarrhea infection has been found to be a carrier for A. baumannii through SIgA appeared to enhance A. baumannii GI tract colonization, the study’s objective was to clarify the relationship between viral and bacterial diarrhea in children with gastroenteritis. Methodology: (50) samples were obtained between the period from (9/2021 to 12/ 2021) in Babylon province. Samples were Stool, blood, and a rectal swab. The virus was diagnosed by chromatographic immunoassay (rapid test) and polymerase chain reaction (PCR)technique to detect the NSP gene in Rotavirus, the bacteria were isolated and diagnosed in the laboratory and a DDT test was performed to detect their sensitivity to the treatment, then PCR was used to diagnose the bacteria through genes assay (Blaoxa-51, INT-2 and MCR- 1) Results: 30 isolates of bacteria out of 50 specimens (60%) were detected. Moreover, (blaOXA-51) gene was investigated by PCR with25 (83%) resultsand play role for identification of A. baumannii, however, Int-Igene was detected in 15 (50%). and Mcr-1 gene was detected in 20(66%) while Rota virus detected by NSP —gene was detected in this study and by the rapid test was found in 20 (40%) PCR- NSP-gene in 15(75%) specimens. This study included the emergence of gastrointestinal tract spread by MDR A. baumannii and Rotavirus. Conclusion: Through this study, it was found that gastroenteritis caused by viruses is more virulent than bacterial infections, so it is recommended to give Roto vaccine to children because it is a major cause of this infection in addition. The possibility of controlling it is possible unlike a viral infection. 

Downloads

Download data is not yet available.

References

Abdul-Hussein TM, Saadedin S, Almaali HMA,

& Al-WattarWM (2019) Evaluation of the

phenotypic and genotypic detection of

Acinetobacter baumannii isolated from

Baghdad hospitals. Plant Archives 19 (2):

-3804. URL:

http://plantarchives.org/19-2/3801-

%20(5154).pdf

Al-Hindawi RA, & Jarallah EM (2018) Detection

of AmpC gene and Some OXA

lactamase class among Carbapenem

Resistant Acinetobacter baumannii

(CRAB) isolates in Hilla, Iraq. Research

Journal of Pharmacy and Technology 11(2): 777-784. DOI:

http://dx.doi.org/10.5958/0974-

X.2018.00147.6

Al-Kadmy IM, Ibrahim SA, Al-Saryi N, Aziz SN,

Besinis A et al. (2020) Prevalence of genes

involved in colistin resistance in

Acinetobacter baumannii: first report

from Iraq. Microbial Drug Resistance 26

(6): 616-622. DOI:

https://doi.org/10.1089/mdr.2019.0243

Al-Saleem N. (2013). Genotyping relatedness of

Acinetobacter baumannii isolated from

medical City/ Baghdad. (Doctoral

dissertation). Baghdad University.

Al-Warid R. (2014). Immunological and Molecular

Study on Acinetobacter baumannii Isolated

from Clinical Samples. (Doctoral

dissertation). University of Babylon.

Amin SU, Alsulaiman M, Muhammad G,

Mekhtiche MA, & Hossain MS (2019)

Deep Learning for EEG motor imagery

classification based on multi-layer CNNs

feature fusion. Future Generation

computer systems 101: 542-554. DOI:

https://doi.org/10.1016/j.future.2019.06.

Badur S, Ozturk S, Pereira P, AbdelGhany M,

Khalaf M et al. (2019) Systematic Review

of the Rotavirus Infection Burden in the

Who-emro Region. Human vaccines &

immunotherapeutics 15 (11): 2754-2768.

DOI:

https://doi.Org/10.1080/21645515.2019.l

Chan AP, Choi Y, Clarke TH, Brinkac LM, White

RC et al. (2020) Abgri4, a Novel

Antibiotic Resistance Island in Multiply

Antibiotic-resistant Acinetobacter

Baumannii Clinical Isolates. Journal of

Antimicrobial Chemotherapy 75 (10):

-2768. DOI:

https://doi.org/10.1093/jac/dkaa266

Fabbretti E, Afrikanova I, Vascotto F, & Burrone

OR (1999) Two non-structural rotavirus

proteins, NSP2 and NSP5, form

viroplasm-like structures in vivo. Journal

of General Virology 80 (2): 333-339. DOI:

https://doi.Org/l0.1099/0022-1317-80-

-333

Garnacho-Montero J, & Timsit J-F (2019)

Managing Acinetobacter baumannii

infections. Current opinion in infectious

diseases 32 (1): 69-76. DOI:

https://doi.org/10.1097/qco.0000000000

Halaji M, Rezaei A, Zalipoor M, & Faghri J

(2018) Investigation of class I, II, and III

integrons among Acinetobacter

Baumannii isolates from hospitalized

patients in Isfahan, Iran. Oman Medical

Journal 33 (1): 37-42. DOI:

https://doi.org/10.5001/omj.2018.07

Hu J, Li G, Wang X, Cai L, Rong M et al. (2021)

Development of a subunit vaccine based on

fiber2 and hexon against fowl adenovirus

serotype 4. Vims Research 305: 198552. DOI:

https://doi.org/10.1016/j.vimsres.2O21.19855

Kareem A (2020) Emerging frontiers in wind

engineering: Computing, stochastics,

machine learning and beyond. Journal of

Wind Engineering and Industrial

Aerodynamics 206: 104320. DOI:

https://doi.org/10.1016/j,jweia.2020.104

Kyriakidis I, Vasileiou E, Pana ZD, & Tragiannidis A

(2021) Acinetobacter baumannii antibiotic

resistance mechanisms. Pathogens 10 (3): 373.

DOI:

https://doi.org/10.3390/pathogensl0030373

Lee Y-T, Kuo S-C, Chiang M-C, Yang S-P, Chen CP et al. (2012) Emergence of carbapenemresistant non-baumannii species of

Acinetobacter harboring a bla OXA-51-like

gene that is intrinsic to A. baumannii.Antimicrobial agents and chemotherapy 56

(2): 1124-1127. DOI:

https://doi.oig/10.1128/aac.00622-11

Liu Y-Y, Wang Y, Walsh TR, Yi L-X, Zhang R et al.

(2016) Emergence of plasmid-mediated

colistin resistance mechanism MCR-1 in

animals and human beings in China: a

microbiological and molecular biological

study. The Lancet infectious diseases 16 (2):

-168. DOI:

https://doi.org/10.1016/S1473-

(15)00424-7

MacFaddin JF (2000) Biochemical Tests for

Identification of Medical Bacteria.

Lippincott Williams & Wilkins,

Philadelphia.

Mirzaei B, Bazgir ZN, Goli HR, Iranpour F,

Mohammadi F et al. (2020) Prevalence of

multi-drug resistant (MDR) and

extensively drug-resistant (XDR)

phenotypes of Pseudomonas aeruginosa

and Acinetobacter baumannii isolated in

clinical samples from Northeast of Iran.

BMC research notes 13: 1-6. DOI:

https://doi.org/10.1186/sl3104-020-

-w

Montero H, Arias CF, & Lopez S (2006)

Rotavirus nonstructural protein NSP3 is

not required for viral protein synthesis.

Journal of virology 80 (18): 9031-9038.

DOI: https://doi.org/10.1128/jvi.00437-

Mshachal MA, Abdulrahman TR, KhudairMS, &

Hassan JS (2017) Molecular detection of

multidrug resistant Acinetobacter

baumannii from different clinical samples.

Iraqi Journal of Medical Sciences 15 (3):

-323. URL:

https://www.iasj.net/iasi?ald=135861&fu

nc=fulltext

Persson BD, John L, Rafie K, Strebl M,

Frangsmyr L et al. (2021) Human species

D adenovirus hexon capsid protein

mediates cell entry through a direct

interaction with CD46. Proceedings of the

National Academy of Sciences 118 (3):

e2020732118. DOI:

https://doi.org/10.1073/pnas.202073211

Peymani A, Farajnia S, Nahaei MR, Sohrabi N,

Abbasi L et al. (2012) Prevalence of class 1

integron among multidrug-resistant

Acinetobacter baumannii in Tabriz,

northwest of Iran. Polish Journal of

Microbiology 61 (1): 57-60. DOI:

https://doi.org/10.33073/pim-2012-007

Rebelo AR, BortolaiaV, Kjeldgaard JS, Pedersen

SK, Leekitcharoenphon P et al. (2018)

Multiplex PCR for detection of plasmidmediated colistin resistance determinants,

mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5

for surveillance purposes.

Eurosurveillance 23 (6): 29-39. DOI:

https://doi.org/10.2807/1560-

ES.2018.23.6.17-00672

Rowe-Magnus DA, & Mazel D (1999) Resistance

gene capture. Current opinion in

microbiology 2 (5): 483-488. DOI:

https://doi.org/10.1016/S1369-

(99)00004-1

StuempfigND, SeroyJ, & Labat-Butler JR(2021)

Viral Gastroenteritis (Nursing). StatPearls

Publishing. URL:

https://pubmed.ncbi.nlm.nih.gov/337604

/

Tate JE, Chitambar S, Esposito DH, Sarkar R,

Gladstone B et al. (2009) Disease and

economic burden of rotavirus diarrhoea in

India. Vaccine 27: F18-F24. DOI:

https://doi.org/10.1016/j.vaccine.2009.0

098

Thirapanmethee K, Srisiri-A-Nun T, Houngsaitong J,

Montakantikul P, Khuntayapom P et al.

(2020) Prevalence of OXA-type -lactamase

genes among carbapenem-resistant

Acinetobacter baumannii clinical isolates inThailand. Antibiotics 9 (12): 864. DOI:

https://doi.oig/10.3390/antibiotics9120864

Turton JF, Woodford N, Glover J, Yarde S,

Kaufmann ME et al. (2006) Identification

of Acinetobacter baumannii by detection

of the bla OXA-51-like carbapenemase

gene intrinsic to this species. Journal of

clinical microbiology 44 (8): 2974-2976.

DOI:

https://doi.org/10.1128/jcm.01021-06

Xu A, Zhu H, Gao B, Weng H, Ding Z et al.

(2020) Diagnosis of severe communityacquired pneumonia caused by

Acinetobacter baumannii through next¬

generation sequencing: a case report.

BMC infectious diseases 20 (1): 1-7. DOI:

https://doi.org/10.1186/sl2879-019-

-5

Yu X, Mullen T-M, Abrishami V, Huiskonen JT,

Nemerow GR et al. (2022) Structure of a

cell entry defective human adenovirus

provides insights into precursor proteins

and capsid maturation. Journal of

Molecular Biology 434 (2): 167350. DOI:

https://doi.Org/10.1016/j.jmb.2021.16735

Zeighami H, Valadkhani F, Shapouri R, Samadi

E, & Haghi F (2019) Virulence

characteristics of multidrug resistant

biofilm forming Acinetobacter baumannii

isolated from intensive care unit patients.

BMC infectious diseases 19 (1): 1-9. DOI:

https://doi.org/10.1186/sl2879-019-

-0

Downloads

Published

2023-02-28

Issue

Section

Articles

How to Cite

Jwan Ahmed Ali , A., & Anwar Kadhim , A.-S. (2023). Relationship between viral and bacterial diarrhea in children suffering from the gastroenteritis infection. History of Medicine, 9(1). http://13.200.237.241/HOM/index.php/medicine/article/view/501